FastQCFastQC Report
Thu 2 Jun 2016
DRR024952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4910363
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138630.2823212866340024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116500.23725333544587232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67860.13819752226057422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90250.053.503641
GTATCAA166050.041.9066471
ATCAACG206900.033.1493643
TCAACGC208250.032.9557044
TATCAAC212050.032.4389042
CAACGCA221050.031.0705535
AACGCAG232850.029.5743566
GTGGTAT40050.027.1187421
ACGCAGA257850.026.4696967
CGCAGAG260000.026.1784958
GTACATG197000.025.8477251
TACATGG197400.024.5806082
TGGTATC43950.024.1774022
GCAGAGT278750.023.8442699
ACATGGG201550.023.3703583
TATACCG4750.020.7833215
GAGTACT170800.020.69271912-13
CAGAGTA271700.020.2329910-11
AGAGTAC258300.020.09065610-11
CATGGGG119000.019.711354
Produced by FastQC (version 0.11.5)