FastQCFastQC Report
Thu 2 Jun 2016
DRR024952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4910363
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159850.3255360143435424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136140.27725037843434386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88760.180760566988632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100150.054.3494641
GTATCAA178200.040.3129121
TATCGCG600.00415860731.339017
ATCAACG229900.030.9224153
TCAACGC231150.030.7307834
TATCAAC233650.030.5078582
CAACGCA241350.029.4515115
AACGCAG253400.028.1252046
GTGGTAT45150.027.5081
TGGTATC48050.025.24672
ACGCAGA287600.024.5339987
GTACATG196600.024.3360631
CGCAGAG289850.024.080878
TACATGG198050.023.0764872
GCAGAGT306500.022.2829
ACATGGG201400.022.0380483
TACCTGG41250.020.63162
GAGTACT193300.020.2032812-13
AGAGTAC284250.020.01393510-11
CAGAGTA298600.019.69741410-11
Produced by FastQC (version 0.11.5)