FastQCFastQC Report
Thu 2 Jun 2016
DRR024951_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024951_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177183
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150350.3599315615332151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124920.29905321361309767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75190.1800016901342364No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88500.057.110381
GTATCAA169950.042.8805581
ATCAACG211150.034.241913
TCAACGC213750.033.8457684
TATCAAC219400.033.065812
CAACGCA226800.031.8161495
GTGGTAT36300.030.8262141
AACGCAG237450.030.5064686
TGGTATC38100.028.135832
ACGCAGA265100.027.147327
CGCAGAG263250.027.0881088
GTACATG200200.026.2559411
TACATGG196800.025.7539332
GCAGAGT278050.024.9193139
TACCTGG34150.024.7817342
ACATGGG202300.024.3765533
GTACCTG40700.021.4866351
GAGTACT170400.021.22415212-13
CAGAGTA273400.021.06146810-11
AGAGTAC263600.020.48031610-11
Produced by FastQC (version 0.11.5)