FastQCFastQC Report
Thu 2 Jun 2016
DRR024951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177183
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163250.3908136176940297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142600.3413783882583071No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93960.2249362788271426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93350.054.6764451
GTATCAA170250.042.524221
GTGGTAT36700.032.685791
ATCAACG219750.032.669323
TCAACGC221650.032.4046674
TATCAAC226150.031.9330332
CAACGCA232150.030.9997675
AACGCAG243300.029.6757076
TGGTATC41500.027.7564282
ACGCAGA276600.025.8808697
GTACATG200500.025.8319851
CGCAGAG275700.025.7248258
TACATGG198750.025.0515382
TACCTGG36900.023.6991162
GCAGAGT293950.023.504399
ACATGGG204150.023.2826143
GTACCTG43100.021.1743131
GAGTACT181600.020.4308712-13
AGAGTAC278350.020.28574210-11
AGTACTT186300.020.1172412-13
Produced by FastQC (version 0.11.5)