FastQCFastQC Report
Thu 2 Jun 2016
DRR024950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205018
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109100.3404037044409735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90540.282494513291345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55640.17360276915761472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65050.051.818611
GTATCAA115050.039.3508031
TACCTGG31250.036.857852
TATAACG3750.032.5953752
GTACCTG36350.032.591931
ATCAACG142900.031.2186433
TCAACGC143850.031.1109964
TATCAAC146600.030.7217162
GTGGTAT29700.030.7085441
ACCTGGG36700.030.2291533
CAACGCA151650.029.758355
AACGCAG158600.028.512266
TGGTATC31150.028.2226312
GTACATG131200.026.2654021
TACATGG130650.025.6922862
ACGCAGA178150.025.1722627
CGCAGAG179200.024.814928
ACATGGG131700.024.7358883
GCAGAGT188300.022.8917359
TAACGCA4300.021.8649444
Produced by FastQC (version 0.11.5)