FastQCFastQC Report
Thu 2 Jun 2016
DRR024950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205018
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123950.38673729757523984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104570.32626961845456093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71580.22333727922900903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69600.052.5193861
GTATCAA119000.039.216841
TACCTGG31750.034.9471472
GTACCTG36600.031.2343921
TATAACG4100.030.9615962
ATCAACG155750.029.7942563
TCAACGC157350.029.456824
TATCAAC159350.029.2686752
CAACGCA164200.028.2852125
AACGCAG171050.027.482276
ACCTGGG39600.027.1884063
GTGGTAT30650.027.0140721
GTACATG125650.026.5829831
TACATGG124850.025.60732
ACAACGC6900.025.2113463
TGGTATC33500.024.7008152
TACAACG6950.023.6772
ACATGGG131250.023.6063863
ACGCAGA197300.023.5864947
CGCAGAG199400.023.2176888
Produced by FastQC (version 0.11.5)