FastQCFastQC Report
Thu 2 Jun 2016
DRR024949_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024949_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4165983
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101380.24335192918454057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90080.21622747860468947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49190.1180753738073343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72000.051.6509551
GTATCAA127650.039.0776981
ATCAACG156400.031.3193823
TCAACGC157400.031.0894184
TATCAAC162400.030.2527352
CAACGCA164250.029.8794175
AACGCAG172000.028.5580376
ACGCAGA191450.025.2151057
CGCAGAG193900.024.7504568
GTACATG120550.023.8291381
GTGGTAT35900.023.0490261
GCAGAGT209050.022.3272069
TACATGG122100.022.101952
TGGTATC36250.022.048312
ACATGGG122450.020.9995883
TACCTGG30050.020.339172
GAGTACT139150.019.77547312-13
AGAGTAC191300.019.6198310-11
CAGAGTA205400.019.46297310-11
TATAACG4001.8189894E-1218.805912
Produced by FastQC (version 0.11.5)