FastQCFastQC Report
Thu 2 Jun 2016
DRR024949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4165983
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107680.25847441048127173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96630.2319500583655766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60430.145055800755788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75000.053.0656971
GTATCAA132350.038.06891
ATCAACG164600.030.5349373
TCAACGC166450.030.1890344
CAACGCA173300.029.1313785
TATCAAC173150.029.0550022
AACGCAG181150.027.8949416
GTACATG123850.025.677661
TATAACG3850.025.6459142
ACGCAGA204500.024.4788367
CGCAGAG204600.024.2569058
GTGGTAT36300.024.234821
TACATGG124350.023.9719332
ACATGGG124900.022.698863
ATAACGC4400.022.4396343
GCAGAGT219750.021.9432419
TGGTATC37950.021.9291152
TACCTGG32800.021.071872
GAGTACT148400.020.40986312-13
AGAGTAC200250.020.3588510-11
Produced by FastQC (version 0.11.5)