FastQCFastQC Report
Thu 2 Jun 2016
DRR024948_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024948_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6358817
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114630.18026938029510833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90120.1417244748512184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91100.050.472681
GTATCAA171700.040.908751
ATCAACG202800.033.9581533
TCAACGC204500.033.7213064
CAACGCA218350.031.5613175
TATCAAC219400.031.5218662
AACGCAG227450.030.4413476
ACGCAGA249200.027.2933797
CGCAGAG250400.026.9742268
GTACATG183600.025.8889451
TACATGG182000.024.5665992
GTGGTAT48600.024.08791
GCAGAGT272200.023.7260369
ACATGGG186300.023.0121043
TGGTATC47450.022.2937012
AGAGTAC249000.020.81145310-11
GAGTACT173400.019.83972412-13
CAGAGTA267750.019.5471710-11
AGTACTT181550.019.18207612-13
CATGGGG106800.019.0583024
Produced by FastQC (version 0.11.5)