FastQCFastQC Report
Thu 2 Jun 2016
DRR024948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6358817
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125420.1972379453599624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107010.16828601923911318No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67040.10542841537977898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92750.051.578021
GTATCAA172700.040.0389371
ATCAACG211850.032.0008853
TCAACGC213500.031.725814
CAACGCA223300.030.2703025
TATCAAC225650.030.2322832
AACGCAG233250.028.9788026
GTGGTAT49500.025.5625781
GTACATG186250.025.5083661
ACGCAGA265700.025.067127
CGCAGAG265800.024.8955978
TGGTATC47750.024.7137812
TACATGG183100.023.8799512
TATACCG5400.023.502645
GCAGAGT293400.022.1211879
ACATGGG188300.022.0962283
AGAGTAC267900.020.06761410-11
GAGTACT187600.019.42620512-13
CAGAGTA286650.018.64021710-11
AGTACTT199150.018.50013212-13
Produced by FastQC (version 0.11.5)