FastQCFastQC Report
Thu 2 Jun 2016
DRR024947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771991
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85220.3074324555887808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65970.2379877856746288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.16782161269643373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54550.052.0552561
TACCTGG29450.041.8252332
GTATCAA101350.041.4238171
GTACCTG31000.040.9472241
GTACCGG3500.038.9540251
TATAACG3450.038.155872
ACCTGGG34200.034.2260253
ATCAACG131250.031.6260223
GTACACG2850.031.3423211
TCAACGC133350.031.0563534
TATCAAC137250.030.3147012
ACAACGC7150.030.2437063
TAACGCA4400.029.9138894
CAACGCA140050.029.7384385
GTGGTAT26400.028.6710991
AACGCAG147850.028.358786
TGGTATC26700.027.292472
GTACATG119300.027.1125531
TACAACG8200.026.9467492
TACATGG115200.026.5675132
Produced by FastQC (version 0.11.5)