FastQCFastQC Report
Thu 2 Jun 2016
DRR024947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771991
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92290.3329375889027057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74420.2684712901304514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55040.19855764322467134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.056.282891
TACCTGG27500.042.9153942
GTATCAA110300.041.8057751
GTACCTG30200.039.7300341
ACAACGC5700.036.29463
ACCTGGG34250.033.2214853
ATCAACG142100.032.4262163
TCAACGC143900.032.0148284
TATCAAC148350.031.2508222
CAACGCA150200.030.734595
AACGCAG158300.029.340126
TATAACG2850.028.0463352
GTGGTAT25700.028.0119921
GTACCGG2700.027.8831581
TGGTATC24700.027.7925242
ACGCAGA174550.026.338397
CGCAGAG175100.026.0098198
GTACAAC11050.025.5490471
TAACGCA3650.024.4707134
GCAGAGT185300.024.1722559
Produced by FastQC (version 0.11.5)