FastQCFastQC Report
Thu 2 Jun 2016
DRR024946_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024946_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3990515
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77860.19511266089715237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67970.1703288923860705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61700.052.4289471
GTATCAA108750.038.2632521
ATCAACG129300.031.2673853
TCAACGC129600.031.1575644
CAACGCA135850.029.6567615
TATCAAC141050.029.201082
AACGCAG141600.028.4178546
GTACATG109850.026.3234581
GTGGTAT30200.025.6888641
ACGCAGA160300.024.8675047
CGCAGAG161000.024.5838938
TACATGG111350.024.5754052
ACATGGG113250.023.2456383
TGGTATC31250.023.020212
GCAGAGT173200.022.2008639
GAGTACT112800.020.24866312-13
CAGAGTA168300.020.0254910-11
AGAGTAC159050.019.9784210-11
TACCTGG28500.019.4672682
GTATAGG14650.018.6147961
Produced by FastQC (version 0.11.5)