FastQCFastQC Report
Thu 2 Jun 2016
DRR024946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3990515
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85300.21375687098031207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75760.18985018224464764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45520.11407048964857919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60450.049.962671
GTATCAA108300.039.094981
ATCAACG131750.031.4407273
TCAACGC133500.030.9883144
CAACGCA139300.029.6980615
TATCAAC142650.029.2694052
AACGCAG146400.028.354126
GTGGTAT30350.027.2810381
GTACATG108750.024.8307341
ACGCAGA167900.024.498397
CGCAGAG169000.024.0024368
TACATGG110550.023.2649082
TGGTATC33450.022.9120642
ACATGGG111700.021.6781063
GCAGAGT183400.021.6565159
AGAGTAC168600.019.73532710-11
GAGTACT121700.019.13398412-13
CAGAGTA179300.018.7672810-11
AGTACTT127500.018.1345712-13
CATGGGG72000.018.0860524
Produced by FastQC (version 0.11.5)