FastQCFastQC Report
Thu 2 Jun 2016
DRR024945_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024945_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4199240
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82950.19753574456330192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70590.16810184700088587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42960.10230422647907716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.052.2482031
GTATCAA121750.040.007351
ATCAACG148800.032.506753
TCAACGC151000.032.0012474
TATCAAC157800.030.7181842
CAACGCA158850.030.5100465
AACGCAG164800.029.5493626
GTACATG143150.025.5855711
ACGCAGA188650.025.3897137
CGCAGAG188950.025.125518
TACATGG143200.024.4600352
ACATGGG147700.023.2965533
GCAGAGT204250.022.4839659
GTGGTAT33850.022.3622861
TACCTGG31650.022.282372
TGGTATC33350.020.582632
GTACCTG35500.020.5282691
GAGTACT122050.020.17758812-13
AGAGTAC185950.020.17167310-11
CATGGGG78650.019.4836184
Produced by FastQC (version 0.11.5)