FastQCFastQC Report
Thu 2 Jun 2016
DRR024945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4199240
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96090.2288271210981035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84380.20094112267934194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53280.1268801021137158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73300.050.632221
GTATCAA134200.041.675741
ATCAACG164500.033.5233
TCAACGC167100.032.940034
TATCAAC172250.032.178852
CAACGCA172500.031.990615
AACGCAG181450.030.490036
CGCAGAG207450.026.3020698
ACGCAGA208850.026.2407687
GTACATG139650.025.9697281
TACATGG142500.024.3476732
GCAGAGT223500.023.929629
ACATGGG143400.023.1454873
GTGGTAT35300.022.9196381
AGAGTAC206600.020.7344410-11
GAGTACT141750.020.60529512-13
CATGGGG77750.020.0715644
TACCTGG34000.020.0495682
TGGTATC36700.019.7274342
CAGAGTA221800.019.63133410-11
Produced by FastQC (version 0.11.5)