FastQCFastQC Report
Thu 2 Jun 2016
DRR024944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932056
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125920.4294597374674972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103220.3520396609068858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68260.2328059218514244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72000.053.0222741
GTATCAA129250.041.6131251
TATAACG2600.032.5487252
ATCAACG167350.031.8544653
TCAACGC169600.031.4862294
TATCAAC169600.031.40792
CAACGCA179250.029.8698275
AACGCAG184200.029.1407136
GTGGTAT30800.027.628841
ACGCAGA203850.026.0089047
GTACATG153950.025.866551
CGCAGAG206850.025.5635228
TACATGG153650.024.96852
TGGTATC34600.024.0509382
GCAGAGT214550.023.8135819
ACATGGG158900.023.0460513
TACAACG6600.022.7950672
TACCTGG27000.022.6367682
TAACGCA3301.8189894E-1221.3670944
GTACCTG30250.021.2926651
Produced by FastQC (version 0.11.5)