FastQCFastQC Report
Thu 2 Jun 2016
DRR024944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932056
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141960.484165377468916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120220.4100194539258459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84510.2882277828254304No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.10774009773346757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76250.055.417151
GTATCAA135300.040.586421
ATCAACG180400.030.288193
TCAACGC181400.030.0637344
TATCAAC183150.029.936622
CAACGCA189400.028.8187055
AACGCAG195750.027.9558966
GTGGTAT31700.026.7190421
TGGTATC33200.025.2111722
GTACATG153250.024.7788221
ACGCAGA221350.024.5510967
TACATGG152950.024.4108072
CGCAGAG224600.023.9401788
TACCTGG27250.022.9507182
GCAGAGT233800.022.5759669
ACATGGG157650.022.5492213
TAACGCA3051.0913936E-1121.5799484
AGAGTAC222150.020.70086910-11
AGTACTT147000.020.63660212-13
GAGTACT146100.020.3133912-13
Produced by FastQC (version 0.11.5)