FastQCFastQC Report
Thu 2 Jun 2016
DRR024943_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024943_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4424494
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105310.23801591775240286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90320.2041363373981296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.12037534687582355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82600.048.8414421
GTATCAA150800.041.126781
ATCAACG183750.033.0821343
TCAACGC186050.032.848934
TATCAAC194950.031.47522
CAACGCA196650.031.0069125
AACGCAG206050.029.7041076
GTACATG168400.027.2234861
ACGCAGA228300.026.582667
TACATGG167800.026.1159132
CGCAGAG230400.026.034038
ACATGGG172900.024.5807863
GCAGAGT245300.023.781959
GTGGTAT41300.023.2253021
TACCTGG32250.021.8696982
TGGTATC44200.020.9568122
AGAGTAC219400.020.84657710-11
CATGGGG95150.020.5043934
GAGTACT145400.020.3313512-13
TAACGCA4450.020.0724874
Produced by FastQC (version 0.11.5)