FastQCFastQC Report
Thu 2 Jun 2016
DRR024943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4424494
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107590.24316904938734238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92700.20951548357846117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59130.13364240069033884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89600.047.5159721
GTATCAA158700.038.5951841
ATCAACG193650.031.2770273
TCAACGC195200.031.0230584
CAACGCA204700.029.5832965
TATCAAC206150.029.5630192
AACGCAG214750.028.2426266
GTACATG170100.026.4118061
TACATGG171400.024.6921422
ACGCAGA244250.024.4831167
CGCAGAG245600.024.1519328
ACATGGG175000.023.3243083
GCAGAGT260850.022.307499
GTGGTAT44750.022.2864991
TACCTGG33000.020.804922
GTACAGG28200.020.0184841
AGAGTAC231700.019.96833610-11
TGGTATC48800.019.6579172
GAGTACT151450.019.56413312-13
CATGGGG99350.018.976894
Produced by FastQC (version 0.11.5)