FastQCFastQC Report
Thu 2 Jun 2016
DRR024942_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024942_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045819
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81730.2683350520828716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69200.22719669159592215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39130.1284711928056132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.051.268551
GTATCAA91950.040.0325741
ATCAACG111250.032.7452353
TCAACGC111550.032.5723574
CAACGCA115900.031.43255
TATCAAC117750.031.0215552
AACGCAG121550.030.0864586
GTACATG90000.027.1621591
CGCAGAG137400.026.1021988
ACGCAGA137800.026.095087
TACATGG88950.025.5272852
TACCTGG27100.025.5006662
GTACCTG29050.025.0835531
ACATGGG90200.024.1800863
GCAGAGT146500.023.9353899
GTGGTAT25600.023.138441
GTCTCGC7150.021.6975841
GAGTACA65350.021.509441
TGGTATC25300.021.3688322
CATGGGG53850.020.8617864
Produced by FastQC (version 0.11.5)