FastQCFastQC Report
Thu 2 Jun 2016
DRR024942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045819
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93430.30674836554634405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80760.26515035857350683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52340.17184212193830298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59000.048.551091
GTATCAA102600.038.0050131
ATCAACG127500.030.271813
TCAACGC128150.030.1167834
TATCAAC133350.029.0857732
CAACGCA133150.029.0211545
AACGCAG140450.027.747056
TACCTGG25750.025.5605682
GTACATG86500.024.7960471
GTACCTG27800.024.5333541
ACGCAGA159950.024.010857
CGCAGAG159600.023.7945218
TGGTATC30450.023.622392
TACATGG85400.023.5616422
GTGGTAT31850.023.1858981
GCAGAGT170500.021.8047229
TATAACG3450.021.8032172
TCTGTCG8700.021.6092268
ACATGGG89450.021.3904293
AGAGTAC154900.019.97869910-11
Produced by FastQC (version 0.11.5)