FastQCFastQC Report
Thu 2 Jun 2016
DRR024941_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024941_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182921
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89640.2816281019855661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75140.2360724629986104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45580.14320179482934073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60400.046.2345241
GTATCAA112000.039.7930451
ATCAACG134000.032.9038433
TCAACGC135700.032.5252574
TATCAAC140150.031.5990962
CAACGCA141300.031.2381765
AACGCAG146300.030.2990916
TACCTGG27000.028.0336062
ACAACGC6150.027.5154113
ACGCAGA162850.027.0755527
CGCAGAG164650.026.5226088
GTGGTAT32600.025.3811871
GTACCTG31750.024.5799621
GCAGAGT172350.024.5194459
GTACATG95650.024.329721
TACAACG6800.023.5064412
TGGTATC32900.023.434992
TACATGG93150.023.1657662
AGAGTAC154250.021.94131910-11
ACATGGG96350.021.8074253
Produced by FastQC (version 0.11.5)