FastQCFastQC Report
Thu 2 Jun 2016
DRR024941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182921
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99850.3137055553687949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86330.2712288492237162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57160.17958347065478533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62050.049.346131
GTATCAA112150.039.7578541
ACAACGC5250.035.8164443
ATCAACG138750.031.8475933
TCAACGC140300.031.426274
TATCAAC142750.031.1213172
CAACGCA146050.030.1890145
TATAACG4200.029.1022342
AACGCAG153400.028.8651086
TACAACG6600.027.7794042
ATAACGC4900.025.9029623
ACGCAGA171450.025.5516687
CGCAGAG171800.025.4424938
TAACGCA4850.025.1987654
GTACATG96100.024.911981
TACCTGG27050.024.3311712
TACATGG94600.023.9032082
GTGGTAT32600.023.805661
GTACCGG2602.7284841E-1123.5171071
GCAGAGT183900.023.2317569
Produced by FastQC (version 0.11.5)