FastQCFastQC Report
Thu 2 Jun 2016
DRR024940_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024940_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343073
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84460.36046678870013865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71590.3055389226029236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.18736078645436996No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46500.053.0900461
TACCTGG29600.050.9941182
GTACCTG33750.045.5596851
GTATCAA88600.041.2376671
ACCTGGG35500.041.0514833
TATAACG3000.037.618092
TAACGCA3350.036.4858134
ATCAACG108400.033.3931163
TCAACGC108800.033.2703484
CAACGCA114250.031.9719815
TATCAAC114000.031.5958942
ACAACGC5100.031.3403783
GTGGTAT20450.030.8118841
AACGCAG120050.030.6198466
GTACCGG2950.030.2857511
GTACCCG2600.028.9369931
TGGTATC22100.027.8731332
ACGCAGA132500.027.2454817
CGCAGAG132850.026.8546878
GTACATG84950.026.7909871
Produced by FastQC (version 0.11.5)