FastQCFastQC Report
Thu 2 Jun 2016
DRR024940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343073
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93040.39708536609828204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78590.33541421884849515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55400.23644163028638032No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46200.057.22691
TACCTGG24800.052.3220862
TATAACG3150.044.7752882
GTACCTG30550.043.4253961
GTATCAA92750.042.8089331
ACCTGGG31700.039.1536563
ACAACGC5050.038.169823
ATCAACG115100.034.2700163
TCAACGC117950.033.4746674
CAACGCA122750.032.3571435
TATCAAC122700.032.3006062
GTACCGG2950.031.8943561
AACGCAG129200.030.8509316
GTGGTAT20750.030.6070581
TGGTATC20000.030.3240622
TAACGCA4450.029.575564
ACGCAGA143150.027.3508017
CGCAGAG143550.027.0758238
GTATAAC6900.025.9083861
GCAGAGT149750.025.452679
Produced by FastQC (version 0.11.5)