FastQCFastQC Report
Thu 2 Jun 2016
DRR024939_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024939_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3042344
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72520.23836883666015413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60610.19922138982311008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39380.12943966888688457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48000.051.9151951
TACCTGG33550.042.7432142
GTATCAA87450.041.4528541
GTACCTG36650.040.1541331
TATAACG3400.040.1031952
ATAACGC3350.037.886633
TCTGTCG11900.035.5524648
ATCAACG108400.032.8271873
TCAACGC110200.032.290464
TATCAAC113450.031.6213152
CAACGCA115350.031.0138425
CTGTCGC14250.030.6790759
TAACGCA4450.030.633594
ACAACGC6300.030.5921733
AACGCAG122200.029.3128456
ACCTGGG50300.027.662463
GTACCGG2800.026.8671631
ACGCAGA134500.026.3875377
CGCAGAG136800.025.8064378
GTCTCGC10950.025.7630351
Produced by FastQC (version 0.11.5)