FastQCFastQC Report
Thu 2 Jun 2016
DRR024939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3042344
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83050.2729803072893795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69960.22995427210072233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48710.16010681237887628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50150.055.447381
TACCTGG33450.042.168642
ACAACGC5700.040.4183463
GTATCAA91750.040.3583261
GTACCTG36950.039.3467181
TATAACG3550.038.4090962
ATCAACG117250.030.9973053
TCAACGC118550.030.6930164
ATAACGC4400.029.9200733
TATCAAC122500.029.8228652
CAACGCA123600.029.5150415
GTGGTAT22950.029.111871
AACGCAG130350.028.2391016
TCTGTCG12700.027.7574238
TGGTATC22400.027.7070522
ACCTGGG51500.026.7496263
TAACGCA5200.026.2177144
GTCTCGC9700.026.1930731
GTCTCAC18900.025.6412931
ACGCAGA146800.024.9782267
Produced by FastQC (version 0.11.5)