FastQCFastQC Report
Thu 2 Jun 2016
DRR024938_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024938_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178986
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200100.6294459931563083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156650.4927671905444063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105230.3310175005489172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61110.19223110765508247No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46850.147374036878426No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.1399817426059756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113800.048.215661
TACCTGG35400.047.8144072
TATAACG3800.042.068292
TACAACG8600.040.4569172
GTATCAA195450.037.7679561
ACCTGGG43550.037.456963
GTACCTG47000.036.8136981
ACAACGC11200.030.640513
ATCAACG244250.030.1018643
TCAACGC247800.029.6886564
TATCAAC251350.029.2374572
ATAACGC5650.029.1213043
CAACGCA260150.028.4242655
AACGCAG270700.027.452396
TAACGCA6250.027.0769654
GTACAAC14100.026.6765961
GTACATG202100.025.963151
GTACCGG2750.025.6459081
TACATGG201150.025.267672
GTATAAC8450.024.4824851
Produced by FastQC (version 0.11.5)