FastQCFastQC Report
Thu 2 Jun 2016
DRR024937_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024937_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679398
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137730.3743275394507471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118820.32293326245217285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74180.20160906757029276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78400.056.077711
GTATCAA141600.041.3760951
TACCTGG34800.032.968962
ATCAACG176000.032.6686943
TCAACGC178100.032.2311364
TATCAAC181450.031.9003772
GTGGTAT32000.031.8863221
CAACGCA186100.030.9466425
AACGCAG195700.029.5943166
TGGTATC33950.029.0853442
GTACCTG45600.026.5010451
ACGCAGA218300.026.2491237
CGCAGAG221600.025.6666158
ACCTGGG43400.025.2396953
GTACATG150500.024.307361
GCAGAGT233250.023.981619
ACAACGC8050.023.3604973
TACATGG149700.023.2122552
ACATGGG153150.021.8565143
CAGAGTA228900.020.86416210-11
Produced by FastQC (version 0.11.5)