FastQCFastQC Report
Thu 2 Jun 2016
DRR024937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679398
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155440.42246041336109874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134420.3653315025990665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90440.24580107941570878No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82650.056.6965331
GTATCAA144300.040.4618261
TATAACG4200.032.4642372
TACCTGG34250.031.1617472
GTGGTAT34100.030.2154981
ATCAACG193450.029.9671173
TCAACGC194700.029.7936044
TATCAAC199600.029.1855092
CAACGCA202650.028.7175835
TGGTATC35150.027.5548732
AACGCAG211550.027.509426
ACAACGC7300.025.7624843
TAACGCA5850.024.9103414
ACCTGGG42800.024.2772273
GTACATG154100.024.0887581
ACGCAGA240400.023.91287
GTACCTG45650.023.9104211
CGCAGAG242800.023.558038
TACATGG152450.023.3775122
ATAACGC6650.022.6244983
Produced by FastQC (version 0.11.5)