FastQCFastQC Report
Thu 2 Jun 2016
DRR024936_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024936_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4428011
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115380.2605684583891052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97070.2192180642731014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57280.12935830556879827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72500.053.433061
GTATCAA135550.040.7876471
ATCAACG166200.032.780043
TCAACGC169450.032.1794324
CAACGCA176300.031.0094665
TATCAAC176650.030.9518742
AACGCAG185650.029.4966936
GTGGTAT34000.028.7610871
TGGTATC33550.027.4653052
ACGCAGA207500.026.163837
CGCAGAG208300.025.8371078
TACCTGG35800.025.6078112
GTACATG132700.024.9060731
GTACCTG39850.023.9490661
TACATGG130050.023.822972
GCAGAGT223200.023.3541989
ACATGGG131050.022.3822983
AGAGTAC208250.020.48279810-11
CAGAGTA221900.019.92182710-11
GAGTACT154000.019.4093112-13
Produced by FastQC (version 0.11.5)