FastQCFastQC Report
Thu 2 Jun 2016
DRR024936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4428011
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128380.2899270123764372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114380.2583101080823873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73020.1649047393965372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79600.053.294261
GTATCAA145600.041.1523861
ATCAACG183650.032.3802723
TCAACGC186200.031.9083334
TATCAAC193350.030.8287532
CAACGCA193200.030.8252245
AACGCAG202750.029.4888656
GTGGTAT34600.028.273151
TATAACG4450.026.4095442
ACGCAGA228500.025.897727
TGGTATC36450.025.793682
CGCAGAG228800.025.574148
TACCTGG37250.024.9873282
GTACCTG40000.023.7508031
GCAGAGT244650.023.3985929
ACAACGC7300.023.1825143
GTACATG128250.023.1030941
TACATGG124250.022.095172
TAACGCA6300.021.6368584
ATAACGC6350.021.468673
Produced by FastQC (version 0.11.5)