FastQCFastQC Report
Thu 2 Jun 2016
DRR024935_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024935_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139114
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85530.2724654154006513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69720.22210088579134113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42210.13446469290379387No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58050.048.836531
GTATCAA100850.040.0914961
TACCTGG31400.035.1858372
GTACCTG34650.032.5639951
ATCAACG125250.031.9753593
TCAACGC126450.031.7085864
TATCAAC130050.030.8367352
CAACGCA131800.030.600775
AACGCAG139000.029.2514886
GTCTCGC8950.028.891451
TCGCTCT9950.027.8724784
ACGCAGA154050.026.0886157
CGCAGAG154150.025.858248
GTACATG85400.024.883431
GTGGTAT28200.024.3407211
ACCTGGG45250.023.4770473
GCAGAGT164600.023.416979
TACATGG89100.023.1641582
GTACACG4300.022.9604571
ACATGGG87850.022.6335243
Produced by FastQC (version 0.11.5)