FastQCFastQC Report
Thu 2 Jun 2016
DRR024935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139114
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96350.3069337399024056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81560.2598185347840187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57750.18396910720668316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58400.050.583521
GTATCAA107350.040.357091
TACCTGG30050.034.88892
ACAACGC6450.032.8004463
ATCAACG136350.031.4460853
TCAACGC137500.031.2118034
TATCAAC139400.030.8255142
GTACCTG35300.030.3824211
CAACGCA143100.030.286015
AACGCAG150600.028.9338056
TATAACG3100.028.8150122
GTGGTAT28250.027.3078581
ACGCAGA168750.025.6821297
CGCAGAG169050.025.3000648
TCTGTCG10600.024.830058
GTACATG86250.024.4877851
TGGTATC30250.024.4006392
GTCTCGC9900.024.2324451
TACAACG8000.024.0946582
GCAGAGT177950.023.3489
Produced by FastQC (version 0.11.5)