FastQCFastQC Report
Thu 2 Jun 2016
DRR024934_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024934_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7990419
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138350.17314486261609058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110610.13842828517503275No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA84540.10580171077386555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144050.045.4647981
GTATCAA244350.040.8676451
TCAACGC323750.030.7224834
ATCAACG325550.030.5539533
TATCAAC334950.029.701142
CAACGCA367750.027.073065
AACGCAG377100.026.4257346
ACGCAGA389050.025.2388677
GTACATG367450.025.218881
CGCAGAG402400.024.1677728
TACATGG367300.023.5779552
GCAGAGT418950.022.4837979
ACATGGG385250.021.8779743
GTGGTAT75200.020.3190271
AGAGTAC379300.019.6415210-11
CATGGGG278750.019.105814
GAGTACA265850.018.9227621
CAGAGTA405900.018.90439810-11
GTACTTT204700.018.83777414-15
GAGTACT210050.018.72755812-13
Produced by FastQC (version 0.11.5)