FastQCFastQC Report
Thu 2 Jun 2016
DRR024934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7990419
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167150.2091880288130072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137740.17238144833205868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86850.10869267306257656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150650.046.4616051
GTATCAA255300.042.064481
ATCAACG341350.031.2919223
TCAACGC344550.031.0245114
TATCAAC355200.030.1648122
CAACGCA375900.028.474595
AACGCAG388600.027.5802886
ACGCAGA421850.025.0600457
CGCAGAG438050.023.9368258
GTACATG368050.023.4525281
GCAGAGT454400.022.7031889
TACATGG372350.021.9572282
ACATGGG391750.020.5215873
AGAGTAC415650.019.89940810-11
GTACTTT230500.019.40962814-15
GAGTACT235650.019.16480612-13
AGTACTT237200.018.94051212-13
CAGAGTA443150.018.7122610-11
CATGGGG287250.018.0338424
GAGTACA275050.017.8545671
Produced by FastQC (version 0.11.5)