FastQCFastQC Report
Thu 2 Jun 2016
DRR024933_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024933_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4040062
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81580.20192759418048534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71550.17710124250568432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40720.10079053242252223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95200.035.9536481
GTATCAA146550.032.467081
ATCAACG168950.027.796233
TCAACGC169350.027.7298894
TATCAAC176650.026.6687452
CAACGCA177150.026.5354655
AACGCAG180950.026.1574696
ACGCAGA198550.023.6733827
CGCAGAG198050.023.6382168
GTACATG90050.023.49511
TACATGG91400.022.016392
GAGTACT130250.021.84846712-13
GCAGAGT214650.021.3721989
ACATGGG93100.021.3079363
AGAGTAC171500.020.30880410-11
TCTGTCG10200.020.276028
GTACTTT151300.018.82357414-15
AGTACTT142300.018.7597912-13
CAGAGTA214900.017.90245210-11
GTCTCGC11600.017.833771
Produced by FastQC (version 0.11.5)