FastQCFastQC Report
Thu 2 Jun 2016
DRR024933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4040062
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90850.2248727866057501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82600.20445230791012614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50270.12442878351866878No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97600.035.041671
GTATCAA155600.032.198841
ATCAACG182550.026.993163
TCAACGC184300.026.6308464
TATCAAC189500.026.152372
CAACGCA190200.025.854195
AACGCAG196450.025.103136
GTACATG92400.023.7763461
CGCAGAG217500.022.4322388
ACGCAGA218050.022.3999867
TACATGG96150.022.0549952
ACATGGG94050.021.5972923
AGAGTAC182850.021.47375710-11
GAGTACT146400.020.51239412-13
GCAGAGT232100.020.4541179
TACAACG5800.019.456422
AGTACTT160100.019.30016312-13
ACAACGC5900.019.1264133
GTACTTT166750.018.16420414-15
CATGGGG59000.017.3705794
Produced by FastQC (version 0.11.5)