FastQCFastQC Report
Thu 2 Jun 2016
DRR024932_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024932_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721948
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128920.47363138458192444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103590.38057303078530524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71150.26139367835094574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39330.14449210638851293No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34140.12542487953480375No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.1019123069213666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68850.051.1440471
GTATCAA124600.040.8627551
TATAACG2950.036.654142
TACCTGG27850.034.2679442
ATCAACG157600.031.8829783
TCAACGC158400.031.6928624
TATCAAC161500.031.2643182
CAACGCA166700.030.1447355
ATAACGC3650.029.6191353
AACGCAG172150.029.3263916
TAACGCA3950.028.560094
GTACCTG34800.028.504961
TACAACG6250.027.8316462
GTGGTAT31750.027.5414261
ACCTGGG35450.027.0490073
TGGTATC33200.026.1969592
ACGCAGA191200.026.132617
CGCAGAG192050.025.8456258
GTACATG128400.025.3737931
TACATGG129700.024.1770362
Produced by FastQC (version 0.11.5)