FastQCFastQC Report
Thu 2 Jun 2016
DRR024932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721948
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141370.5193706859940014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120980.44446109918337895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91340.3355684972673982No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38540.14158977320654179No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34270.12590247866601417No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33570.12333079103641952No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73350.052.32341
GTATCAA127600.039.3296131
ACAACGC7100.036.415543
TATAACG2850.036.289772
TACAACG7100.033.7689972
TACCTGG25150.033.08582
ATAACGC3850.030.5254233
TAACGCA3100.030.3268134
TATCAAC168500.029.6299512
ATCAACG169750.029.3547573
TCAACGC172550.028.9040644
CAACGCA180200.027.7813455
AACGCAG186750.026.9076396
GTGGTAT33750.026.6173921
ACCTGGG31600.025.140973
GTACCTG34400.025.0206491
GTACAAC11300.024.9734611
TGGTATC35500.024.896362
GTACATG125100.023.6106661
ACGCAGA211950.023.484497
Produced by FastQC (version 0.11.5)