FastQCFastQC Report
Thu 2 Jun 2016
DRR024931_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024931_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388561
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105050.31001360164388364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93260.2752200712927995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56720.16738668715127158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75650.045.3048251
GTATCAA129150.036.256911
ATCAACG156000.029.8920153
TCAACGC157000.029.7606284
TATCAAC159950.029.2164842
CAACGCA164750.028.4475125
AACGCAG169750.027.5802696
GTACATG115250.025.9035111
TACATGG114000.024.9915752
ACGCAGA187950.024.6837397
CGCAGAG189300.024.4083828
GTGGTAT41950.023.8711571
ACATGGG116800.023.2623273
GCAGAGT201300.022.5096829
TGGTATC41950.022.0780182
TACCTGG25450.021.6134412
GAGTACT130600.020.31477412-13
CCGGTCG3052.401066E-1020.0345529
AGAGTAC178900.019.75802810-11
CAGAGTA200400.019.23323210-11
Produced by FastQC (version 0.11.5)