FastQCFastQC Report
Thu 2 Jun 2016
DRR024931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388561
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117830.34772872614658557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106630.31467634786565746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70850.20908580367890675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80950.048.925971
GTATCAA135300.036.3297731
ATCAACG168950.028.8006273
TCAACGC170450.028.5438044
TATCAAC173600.028.0562612
CAACGCA176050.027.662555
AACGCAG182350.026.7841766
ACGCAGA208350.023.2599097
GTACATG110700.023.1575741
CGCAGAG209650.023.00128
TAGACCG1652.6626203E-622.7915845
TACATGG111300.021.9648362
TACCTGG27350.021.8306242
GCAGAGT221300.021.2593849
GAGTACT149100.020.67691412-13
ACATGGG116150.020.1976623
AGAGTAC198000.020.16356710-11
GTGGTAT40050.019.7310851
AGTACTT151200.019.50389912-13
CAGAGTA218500.019.30421610-11
Produced by FastQC (version 0.11.5)