FastQCFastQC Report
Thu 2 Jun 2016
DRR024930_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024930_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3397666
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104490.3075346428989783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88840.26147361159101573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53290.15684296219816782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67850.053.6245461
GTATCAA125200.040.2872771
ATCAACG153200.032.7083133
TCAACGC155450.032.2953724
TATCAAC161250.031.1055032
CAACGCA163950.030.5923425
AACGCAG170200.029.5781146
GTGGTAT30950.028.098531
TGGTATC30950.027.4909932
TATAACG3800.027.2152042
ACGCAGA187700.026.5700077
CGCAGAG188600.026.4428258
GTACATG105700.025.438621
TACATGG104250.024.2593272
GCAGAGT200350.024.258589
TACCTGG26950.023.547652
ACATGGG107250.022.0022033
ATAACGC4750.021.7715233
GTACCTG30250.021.7558061
AGAGTAC183400.021.4003710-11
Produced by FastQC (version 0.11.5)