FastQCFastQC Report
Thu 2 Jun 2016
DRR024930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3397666
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111970.32954975562636235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100950.2971157259130238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65300.19219075683130712No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71050.053.5562251
GTATCAA126850.039.156061
ACAACGC5450.031.0500973
ATCAACG160550.030.5958793
TCAACGC163400.030.088344
TATCAAC169250.029.1637362
CAACGCA170050.028.8840625
AACGCAG178150.027.6235476
TGGTATC31500.026.862382
GTGGTAT33850.026.12311
TACCTGG28050.024.6358182
ACGCAGA202350.024.1801917
CGCAGAG202100.024.0680838
GTACATG106800.023.2534921
GTACCTG31700.022.7016621
TACATGG103700.022.4846152
GCAGAGT212900.022.2952929
ACATGGG107800.020.7560713
AGAGTAC198750.020.53667410-11
TATAACG2801.7971615E-920.1467842
Produced by FastQC (version 0.11.5)