FastQCFastQC Report
Thu 2 Jun 2016
DRR024929_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024929_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7316829
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140150.19154472518081261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109010.14898530497296028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118500.054.119371
GTATCAA224600.043.751531
ATCAACG288000.033.6896673
TCAACGC292650.033.1695184
TATCAAC307750.031.7929462
CAACGCA325450.029.8131545
AACGCAG339050.028.6987086
ACGCAGA360650.026.6142187
CGCAGAG372050.025.659588
GTGGTAT47550.024.6209931
GTACATG355650.024.510021
GCAGAGT385550.024.066199
TACATGG346500.023.9360282
ACATGGG367250.021.926793
TGGTATC56700.020.3160722
AGAGTAC372400.019.98076810-11
CAGAGTA386350.019.56347710-11
CATGGGG237300.019.4131494
GTACTTT191150.019.33676114-15
AGTACTT198300.018.86559112-13
Produced by FastQC (version 0.11.5)