FastQCFastQC Report
Thu 2 Jun 2016
DRR024929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7316829
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155990.2131934475986797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127430.1741601450573739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91940.12565552645825126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116500.054.1511651
GTATCAA224100.043.664291
ATCAACG297500.032.4457243
TCAACGC302450.031.9412084
TATCAAC316300.030.7259752
CAACGCA324850.029.7387075
AACGCAG338300.028.695336
GTGGTAT46400.027.4761751
ACGCAGA382250.025.1613227
TGGTATC51100.024.8431852
GTACATG352100.024.3971861
CGCAGAG393550.024.3043168
TACATGG343200.023.7693122
GCAGAGT409250.022.7287759
ACATGGG366900.021.682443
AGAGTAC395600.019.809310-11
AGTACTT219800.019.13564312-13
CATGGGG241600.018.8547154
GTACTTT215150.018.62096614-15
CAGAGTA411250.018.4955110-11
Produced by FastQC (version 0.11.5)