FastQCFastQC Report
Thu 2 Jun 2016
DRR024928_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024928_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4951584
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158330.3197562638541525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130530.2636126136605983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79120.15978725191777016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93350.057.8637541
GTATCAA182600.044.333631
ATCAACG229650.035.0632633
TCAACGC231500.034.8019564
TATCAAC238000.033.8559272
CAACGCA243300.033.0950855
AACGCAG253600.031.9531976
GTGGTAT40150.028.335491
ACGCAGA287950.027.847357
CGCAGAG289650.027.5540928
TGGTATC39950.027.5359422
GTACATG215900.026.7173161
TACATGG214900.025.682222
GCAGAGT309350.025.0852789
ACATGGG221850.024.0702343
GAGTACT189350.021.71837812-13
CAGAGTA300750.021.22345410-11
AGAGTAC289300.020.65807510-11
CATGGGG114550.020.6403374
GTACTTT204750.020.00391414-15
Produced by FastQC (version 0.11.5)