FastQCFastQC Report
Thu 2 Jun 2016
DRR024928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4951584
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178810.3611167658672457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149920.3027717998927212No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102870.20775170127377418No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98050.061.693561
GTATCAA187700.042.602111
ATCAACG241200.032.8018843
TCAACGC244600.032.3205074
TATCAAC250350.031.621792
CAACGCA254400.031.1493595
GTGGTAT38500.030.9105171
AACGCAG264750.029.984886
TGGTATC39800.027.9935022
GTACATG213400.026.825181
ACGCAGA304300.025.8076257
TACATGG216950.025.6990472
CGCAGAG306350.025.5275448
ACATGGG221450.024.2852343
GCAGAGT326500.023.4051239
GAGTACT218000.021.08371512-13
CAGAGTA321050.020.80851710-11
AGAGTAC311950.020.31575410-11
CATGGGA97900.019.8278664
GTACTTT228700.019.75822414-15
Produced by FastQC (version 0.11.5)