FastQCFastQC Report
Thu 2 Jun 2016
DRR024927_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024927_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640816
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88780.24384643442568915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78740.21627019876862769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44580.12244507824619535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58200.057.5135231
GTATCAA113150.042.2966731
ATCAACG141000.033.6390153
TCAACGC143250.033.2405474
TATCAAC148350.031.9120662
CAACGCA151400.031.5438865
GTGGTAT25800.030.4305461
AACGCAG158300.030.3763876
TGGTATC25150.028.9738832
CGCAGAG173900.027.4077768
ACGCAGA174300.027.3988137
GTACATG102900.025.0824491
TACATGG97500.025.0250932
GCAGAGT188550.024.8045859
TATAACG2352.0190782E-1024.0063632
ACATGGG102750.022.5089113
AGAGTAC180250.021.38326510-11
ACAACGC6600.021.3672473
GTCTCGC11750.021.205621
CAGAGTA189450.020.63018210-11
Produced by FastQC (version 0.11.5)